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Keywords brevibacterium flavum;L-leucine;auxotroph;fermentation; 黄色短杆菌;L-亮氨酸;营养缺陷型;发酵;
Keywords Brevibacterium flavum;L-leucine;fermentation condition; 黄色短杆菌;L-亮氨酸;发酵条件;
Keywords Brevibacterium flavum;Protoplast;Generation quotiety;Regeneration quotiety;Fusion; 黄色短杆菌;原生质体;形成率;再生率;融合;
In this study the SerB gene was obtained from E. coli Jm109 by PCR and inserted into pEC7 and this recombinant plasmids with SerB gene were transformed into Brevibacterium flavum C-l1 (BfC-11 ) by the method of electrotransformation. 本研究应用PCR技术从大肠杆菌JM109(E.;coli JM109)中扩增出磷酸丝氨酸转氨酶基因，将其与表达载体pEC 7连接。
Keywords L-leucine;pathway analysis;Brevibacterium flavum; 亮氨酸;途径分析;黄色短杆菌;
Objective:Using Brevibacterium flavum XV0505 as production strain,the influence of fermentation medium and fermentation conditions on the yield and glucose acid invert ratio of L-valine were studied. 目的:以黄色短杆菌XV0505为生产菌株,探讨发酵培养基和发酵控制条件对L-缬氨酸的产量和糖酸转化率的影响。
Keywords L valine;breeding;fermentation;Brevibacterium flavum; 缬氨酸;诱变育种;发酵;黄色短杆菌;
Keywords L-Isoleucine;Kla;batch fermentation;Brevibacterium flavum; 异亮氨酸;体积传氧系数;分批发酵;黄色短杆菌;
Keywords L-isoleucine;batch fermentation;kinetic model;Brevibacterium flavum; 异亮氨酸;分批发酵;动力学模型;黄色短杆菌;
Keywords NaCS-PDMDAAC microcapsules;immobilization;Brevibacterium flavum; PDMDAAC微胶囊;固定化;黄色短杆菌;
Keywords amino acid;fermentation;L-arginine;Brevibacterium flavum;breeding; 氨基酸;发酵;L-精氨酸;黄色短杆菌;诱变育种;
Keywords L-valine;Brevibacterium flavum;protoplast fusion;fermentation; 缬氨酸;黄色短杆菌;原生质体融合;发酵;
Keywords environmental factors;L-isoleucine;Brevibacterium flavum;batch fermentation; 环境因素;L-异亮氨酸;黄色短杆菌;分阶段发酵;
Keywords L-leucine;metabolic flux analysis;linear program;Brevibacterium flavum; 亮氨酸;代谢流分析;线性规划;黄色短杆菌;
Keywords L-leucine,,,,, Brevibacterium flavum,,,,, Mutagenesis breeding,,,,, Fermentation; 亮氨酸;黄色短杆菌;诱变育种;发酵;
Keywords Brevibacterium ammoniagenes;fermentation;inosine; 产氨短杆菌;发酵;肌苷;
Keywords L-isoleucine;Brevibacterium flavum;mutagenic breeding;protoplast fusion;batch fermentation; 关键词L-异亮氨酸;黄色短杆菌;诱变育种;原生质体融合;分批发酵;
Keywords L-valine,Brevibacterium flavum,protoplast mutagenesis,neural network,batch fermentation kinetics; 缬氨酸;黄色短杆菌;原生质诱变;神经网络;分批发酵动力学;
Keywords L-valine, Brevibacterium flavum, metabolic flux, batch fermentation, fed batch fermentation, fermentation kinetics, membrane filtration.; 缬氨酸;黄色短杆菌;代谢流;分批发酵;补料分批发酵;发酵动力学;膜过滤;
Keywords Brevibacterium ammoniagenes;Inosine;Glutamic acid mother liquid; 产氨短杆菌;肌苷;味精母液;